A retrospective examination (Journal of Occupational Medicine and Toxicology, Oct 2011) was conducted of injuries, physical fitness, and their association among Federal Bureau of Investigation (FBI) new agent trainees. Injuries and activities associated with injuries were obtained from a review of medical records in the medical clinic that served the new agents. A physical fitness test (PFT) was administered at Weeks 1, 7 and 14 of the 17-week new agent training course. The PFT consisted of push-ups to exhaustion, 1-minute bent-leg sit-ups, 300-meter sprint, a 1.5-mile run, and pull-ups to exhaustion. Among both men and women, higher injury incidence was associated with lower performance on any of the physical fitness measures.